Intracellular Vesicle Rupture Common Amyloid Protein Mechanism
Abnormal proteins found in Alzheimer’s disease, Parkinson’s disease, and Huntington’s disease all share a similar ability to cause damage when they invade brain cells, a Loyola University Chicago study has found. The finding potentially could explain the mechanism by which Alzheimer’s, Parkinson’s, Huntington’s, and other neurodegenerative diseases spread within the brain and disrupt normal brain functions.
The finding also suggests that an effective treatment for one neurodegenerative disease might work for other neurodegenerative diseases as well.
“A possible therapy would involve boosting a brain cell’s ability to degrade a clump of proteins and damaged vesicles. If we could do this in one disease, it’s a good bet the therapy would be effective in the other two diseases,”
Neurodegenerative diseases are caused by the death of neurons and other cells in the brain, with different diseases affecting different regions of the brain. Alzheimer’s destroys memory, while Parkinson’s and Huntington’s affect movement. All three diseases are progressive, debilitating and incurable.
Previous research has suggested that in all three diseases, proteins that are folded abnormally form clumps inside brain cells. These clumps spread from cell to cell, eventually leading to cell deaths.
Different proteins are implicated in each disease: tau in Alzheimer’s, alpha-synuclein in Parkinson’s and huntingtin in Huntington’s disease.
The Loyola study focused on how these misfolded protein clumps invade a healthy brain cell. The authors observed that once proteins get inside the cell, they enter vesicles (small compartments that are encased in membranes).
The proteins damage or rupture the vesicle membranes, allowing the proteins to then invade the cytoplasm and cause additional dysfunction. (The cytoplasm is the part of the cell that’s outside the nucleus).
Like Digesting Nails
The Loyola study also showed how a cell responds when protein clumps invade vesicles: The cell gathers the ruptured vesicles and protein clumps together so the vesicles and proteins can be destroyed. However, the proteins are resistant to degradation.
“The cell’s attempt to degrade the proteins is somewhat like a stomach trying to digest a clump of nails,” Campbell said.
First author William Flavin, PhD, said the finding that protein clumps associated with the three diseases cause the same type of vesicle damage was unexpected. Loyola researchers initially focused on alpha-synuclein proteins associated with Parkinson’s disease.
So they asked collaborator Ronald Melki, PhD, to send them samples of different types of alpha-synuclein. To do the experiment in a blinded, unbiased manner, the Loyola researchers did not know which types of alpha-synuclein were which.
Melki, a protein researcher at the Paris-Saclay Institute of Neuroscience, is known for his ability to generate distinct types of alpha-synuclein. Without telling the Loyola researchers, Melki sent other types of proteins as well. This led to the surprise finding that tau and huntingtin proteins also can damage vesicles.
The study’s findings need to be followed up and confirmed in future studies, the researchers noted.
Image: The fusion of ruptured vesicles containing alpha-synuclein protein (green) leads to the formation of large protein clumps resembling the proteins that form in the brains of Parkinson’s disease patients. Credit: Loyola University Chicago