The gene-editing tool CRISPR-Cas9 holds promise for correcting defects inside individual cells and potentially healing or preventing many human ailments. But the Cas9 system alters DNA, not RNA, and some experts believe that being able to modify RNA ultimately may prove just as useful.

Now, scientists from the Salk Institute are reporting for the first time the detailed molecular structure of CRISPR-Cas13d, a promising enzyme for emerging RNA-editing technology. They were able to visualize the enzyme thanks to cryo-electron microscopy (cryo-EM), a cutting-edge technology that enables researchers to capture the structure of complex molecules in unprecedented detail.

“This paper provides a molecular blueprint for RNA-targeted genetic engineering. It adds to the breadth of tools that are needed for conducting this kind of crucial biomedical research,”

says Salk Assistant Professor Dmitry Lyumkis, a structural biologist and one of the corresponding authors of the study.

Controlling RNA

Derived from genes originally found in bacteria, CRISPR has been described as “molecular scissors” or a “word processing program for living cells.” It swaps out one segment of genetic code with another.

In the CRISPR-Cas9 system, Cas9 is the enzyme that cuts DNA. Having editing tools for RNA, however, would allow scientists to modify a gene’s activity without making a permanent — and potentially dangerous — change to the gene itself.

“DNA is constant, but what’s always changing are the RNA messages that are copied from the DNA. Being able to modulate those messages by directly controlling the RNA has important implications for influencing a cell’s fate,”

says Salk Research Associate Silvana Konermann, a Howard Hughes Medical Institute Hanna Gray Fellow and one of the study’s first authors.

CRISPR-Cas13d

Earlier this year, Konermann and Helmsley-Salk Fellow Patrick Hsu published a paper in Cell discovering the family of enzymes called CRISPR-Cas13d and reporting that this alternate CRISPR system was effective in recognizing and cutting RNA. The team also showed this tool could be used to correct a disease-causing protein imbalance in cells from a person with dementia.

The new study, a collaboration between the Lyumkis and Hsu labs, built on the discovery of the Cas13d family and provides the molecular details that explain how it works.

“In our previous paper, we discovered a new CRISPR family that can be used to engineer RNA directly inside of human cells. Now that we’ve been able to visualize the structure of Cas13d, we can see in more detail how the enzyme is guided to the RNA and how it is able to cut the RNA. These insights are allowing us to improve the system and make the process more effective, paving the way for new strategies to treat RNA-based diseases,”

says Hsu, who is the other corresponding author of the new work.

The team used cryo-EM to reveal new details into Cas13d by freezing the enzyme in different, dynamic states, allowing researchers to decode a range of activities instead of just seeing one activity at a single point in time.

“This enabled us to see how Cas13d guides, binds and targets the RNA. We hope this new knowledge will help expand on the power of gene-editing tools,”

explains Cheng Zhang, a research associate in the Lyumkis lab and the paper’s other first author.

Cheng Zhang, Silvana Konermann, Nicholas J. Brideau, Peter Lotfy, Xuebing Wu, Scott J. Novick, Timothy Strutzenberg, Patrick R. Griffin, Patrick D. Hsu, Dmitry Lyumkis
Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d
Cell Volume 175, Issue 1, P212-223.E17, September 20, 2018