Spinal Interneurons Inhibit Distracting Input To Enable Smooth Walking

Specific neurons called RORbeta (RORβ) interneurons inhibit transmission of potentially disruptive sensory information during walking in order to promote a fluid gait, Salk Institute scientists have found.

The research solves a longstanding mystery about how our spinal cord knows when to pay attention to certain pieces of information, and when to ignore it as distracting from the task at hand, and illustrates a high level of sophistication in spinal cord information processing.

We think of our brain as masterminding all of our actions, but a surprising amount of information related to movement gets processed by our spinal cord.

“This research provides a sense of how the nervous system deals with the different types of information that’s coming in to it, and how it uses that information in a way that’s relevant to what it’s actually doing at the time. Your spinal cord is incredibly smart,”

says Martyn Goulding, a professor in Salk’s Molecular Neurobiology Laboratory.

Spinal Cord Traffic

When we are moving, motor circuits in the spinal cord are constantly being barraged by information from sensory receptors in the skin and muscles, telling these circuits what our limbs are doing or what the ground underfoot feels like. This information is critical for actions like walking or standing still.

Often these actions are at odds with each other, so a big question in neuroscience has been how our spinal cord “gates” or traffics different kinds of sensory information that might cause conflicting actions, to ensure that each movement is performed properly.

Goulding’s team discovered that a special set of “middle man” neurons (interneurons) inhibit conflicting sensory information that comes primarily from muscles to prevent it from triggering responses in motor neurons that would lead to conflicting actions. This type of inhibition is termed presynaptic inhibition, because it occurs before the signal is passed across the synaptic gap to neurons on the other side.

The team was led to explore these neurons by earlier experiments by other researchers who had mutated the RORβ gene and found that mice with the mutation had an abnormal duck-like gait.

But because RORβ, a regulatory protein known as a transcription factor, is expressed by cells in the brain and in different parts of the spinal cord, it wasn’t clear which location was responsible for the duck gait.

RORbeta Interneurons

The Goulding lab undertook a series of experiments to isolate the location of the defect using genetic and molecular strategies to disable the RORβ gene in various types of neurons and ask what happens. The duck gait only appeared when they inactivated RORβ inhibitory cells in the dorsal spinal cord.

Cells in the dorsal spinal cord (the back) receive sensory information from the body and then pass it on to neurons in the ventral part of the spinal cord (the front) that generate coordinated movement. In mice that lacked functional RORβ interneurons, the motor neurons that cause their limbs to flex remained active, causing their gait to become duck-like and abnormal.

This means that RORβ interneurons are gating — inhibiting — irrelevant sensory information that would interfere with the normal stepping pattern. When RORβ is present, each step is a smooth fluid motion, but when absent, the legs become excessively flexed (bent) and each step is awkward.

In humans this would be akin to your knee continuing to stay bent for too long with each step.

Understanding Fundamentals

Stephanie Koch, a Salk research associate and first author of the paper, said:

“I think what’s really exciting about this project is that we managed to isolate and describe this very local and very specific circuit, which is only active during stepping.”

These results add to other work by the lab examining how specific interneurons in the spinal cord are responsible for gating light touch, and getting rid of them causes hypersensitivity and chronic itch. Mice with this mutation could walk perfectly, but scratched excessively.

Together these findings support the idea that there are dedicated populations of inhibitory interneurons in the nervous system that selectively shut off certain types of incoming information when it’s not relevant to the task at hand (for instance, walking smoothly and not scratching when there’s nothing lightly touching you).

“We’re trying to understand how the nervous system fundamentally works. Once you understand that, you can start to look at medical or clinical issues that are related to it,”

says Goulding, who holds the Frederick W. and Joanna J. Mitchell Chair at Salk.

Koch, Stephanie C. et al.
RORβ Spinal Interneurons Gate Sensory Transmission during Locomotion to Secure a Fluid Walking Gait
Neuron DOI: http://dx.doi.org/10.1016/j.neuron.2017.11.011