Schizophrenia Patients’ Blood Has Higher Microbial Diversity

The blood of schizophrenia patients has genetic material from more types of microorganisms than that of people without the debilitating mental illness, Oregon State University researchers have found. It is unknown whether this is a cause or an effect of the severe, chronic condition that strikes about one person in 100.

“It’s a common assumption that healthy blood is sterile so some may find it surprising that we even found bacterial genetic material in the bloodstream,”

said David Koslicki, a mathematical biologist in the OSU College of Science.

Koslicki and collaborators performed whole-blood transcriptome analyses on 192 people. Subjects included healthy controls as well as those with schizophrenia, bipolar disorder and amyotrophic lateral sclerosis, or Lou Gehrig’s disease.

Transcriptome Analysis

Transcriptome analysis is the sequencing of ribonucleic acid, or RNA. RNA works with DNA, the other nucleic acid — so named because they were first discovered in the cell nuclei of living things — to produce the proteins needed throughout an organism.

Microbial profiling using RNA-Seq data from whole blood.

Microbial profiling using RNA-Seq data from whole blood.
Credit: Loes M. Olde Loohuis, et al CC-BY

Studying the blood samples, researchers detected RNA from a range of bacteria and archaea — with the range wider for schizophrenics than for the other three groups.

“More and more we know the human microbiome plays a huge role in health and disease. Gut bacteria account for most of the trillions of microbial cells in the human body, and this study shows that microbiota in the blood are similar to ones in the mouth and gut. It appears there’s some sort of permeability there into the bloodstream. Down the road, microscopy, culturing or direct measures of permeability may be able to shed light on that,”

Koslicki said.

Elevated Microbial Diversity

Researchers found schizophrenics’ blood samples were more likely to contain two phyla in particular — Planctomycetes and Thermotogae — but the overall results suggest it’s not likely those are the sole reason for the elevated microbial diversity.

Phylogenetic classification is performed using PhyloSift, which is able to assign the filtered candidate microbial reads to the microbial genes from 23 distinct taxa on the phylum level

Phylogenetic classification is performed using PhyloSift, which is able to assign the filtered candidate microbial reads to the microbial genes from 23 distinct taxa on the phylum level.
Credit: Loes M. Olde Loohuis, et al CC-BY

Scientists also found fewer of the immunity-enhancing CD8+ memory T cells in the blood of schizophrenics.

“That could suggest a mechanism for why we see increased microbial diversity in the blood, and it’s also possibly affected by lifestyle or health status differences between schizophrenia patients and the other groups,” Koslicki said. “In addition, it’s interesting that bipolar disorder, which is genetically and clinically correlated to schizophrenia, didn’t show a similar increased microbial diversity.”

Koslicki also notes that the RNA sequencing can’t detect if microbes are actually living in the blood, only that their genetic material is there; it could have gotten there from somewhere else in the body.

The research was supported by the National Institute of Neurological Disorders And Stroke and the National Institutes of Health.

Loes M. Olde Loohuis, Serghei Mangul, Anil P. S. Ori, Guillaume Jospin, David Koslicki, Harry Taegyun Yang, Timothy Wu, Marco P. Boks, Catherine Lomen-Hoerth, Martina Wiedau-Pazos, Rita M. Cantor, Willem M. de Vos, René S. Kahn, Eleazar Eskin & Roel A. Ophoff
Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia
Translational Psychiatry volume 8, Article number: 96 (2018) doi:10.1038/s41398-018-0107-9

Top Image: Daniel Friedman/Flickr