Optogenetics Using NanoLuc Ratiometric Bioluminescent Sensor
A new form of bioluminescent stimulation causes individual brain cells to imitate fireflies and glow in the dark. Scientists at Vanderbilt University created the technique as a new and improved method for tracking the interactions within large neural networks in the brain.
The probe is a genetically modified form of luciferase, the enzyme that a number of other species including fireflies use to produce light.
Carl Johnson, Stevenson Professor of Biological Sciences, who headed the effort, explains:
“For a long time neuroscientists relied on electrical techniques for recording the activity of neurons. These are very good at monitoring individual neurons but are limited to small numbers of neurons. The new wave is to use optical techniques to record the activity of hundreds of neurons at the same time.
Most of the efforts in optical recording use fluorescence, but this requires a strong external light source which can cause the tissue to heat up and can interfere with some biological processes, particularly those that are light sensitive.”
NanoLuc Luminescence Plus Optogenetics
Based on their research on bioluminescence in “a scummy little organism, the green alga Chlamydomonas, that nobody cares much about” Johnson and his colleagues realized that if they could combine luminescence with optogenetics, a new biological technique that uses light to control cells, particularly neurons, in living tissue, they could create a powerful new tool for studying brain activity.
“There is an inherent conflict between fluorescent techniques and optogenetics. The light required to produce the fluorescence interferes with the light required to control the cells,” said Johnson. “Luminescence, on the other hand, works in the dark!”
The ideal sensor would have no autofluorescence, photobleaching, phototoxicity or photoresponse. It would also be ratiometric.
As the authors write in the paper:
“ratiometric sensors monitor an ionic concentration independently of the intracellular concentration of the sensor (that is, expression level for a genetically encoded sensor) because the measured quantity is the ratio of two wavelengths. Ratiometric recording is less influenced by changes in optical path length, and it also helps to cancel movement and growth artifacts.”
Johnson collaborated with colleagues Associate Professor Donna Webb, Research Assistant Professor Shuqun Shi, post-doctoral student Jie Yang and doctoral student Derrick Cumberbatch in biological sciences and Professor Danny Winder and postdoctoral student Samuel Centanni in molecular physiology and biophysics.
The team genetically modified a type of luciferase obtained from Oplophorus gracilirostris, a luminescent species of shrimp, so that it would light up when exposed to calcium ions. Then they hijacked a virus that infects neurons and attached it to their sensor molecule so that the sensors are inserted into the cell interior.
The researchers picked calcium ions because they are involved in neuron activation.
Although calcium levels are high in the surrounding area, normally they are very low inside the neurons. However, the internal calcium level spikes briefly when a neuron receives an impulse from one of its neighbors.
They tested their new calcium sensor with one of the optogenetic probes (channelrhodopsin) that causes the calcium ion channels in the neuron’s outer membrane to open, flooding the cell with calcium. Using neurons grown in culture they found that the luminescent enzyme reacted visibly to the influx of calcium produced when the probe was stimulated by brief light flashes of visible light.
To determine how well their sensor works with larger numbers of neurons, they inserted it into brain slices from the mouse hippocampus that contain thousands of neurons.
In this case they flooded the slices with an increased concentration of potassium ions, which causes the cell’s ion channels to open. Again, they found that the sensor responded to the variations in calcium concentrations by brightening and dimming.
“We’ve shown that the approach works,” Johnson said. “Now we have to determine how sensitive it is. We have some indications that it is sensitive enough to detect the firing of individual neurons, but we have to run more tests to determine if it actually has this capability.”