Endocannabinoid Epoxides May Have Anti-cancer Effects

Endocannabinoids, a class of molecules formed when the body metabolizes omega-3 fatty acids, could inhibit cancer’s growth and spread, University of Illinois researchers report in a new study in mice. Endocannabinoids are made naturally by the body and have similar properties to cannabinoids found in marijuana – but without the psychotropic effects.

In mice with tumors of osteosarcoma – a bone cancer that is notoriously painful and difficult to treat – endocannabinoids slowed the growth of tumors and blood vessels, inhibited the cancer cells from migrating and caused cancer cell death.

“We have a built-in endocannabinoid system which is anti-inflammatory and pain-reducing. Now we see it is also anti-cancer, stopping the cells from proliferating or migrating. These molecules could address multiple problems: cancer, inflammation and pain,”

said study leader Aditi Das, a professor of comparative biosciences and an affiliate of biochemistry at Illinois.

Endocannabinoid Epoxides

In 2017, the Illinois team identified a new group of omega-3 fatty-acid metabolites called endocannabinoid epoxides, or EDP-EAs. They found that these molecules had anti-inflammatory properties and targeted the same receptor in the body that cannabis does.

Since cannabis has been shown to have some anti-cancer properties, in the new study the researchers investigated whether EDP-EAs also affect cancer cells. They found that in mice with osteosarcoma tumors that metastasized to their lungs, there was an 80 percent increase in naturally occurring EDP-EAs in cancerous lung tissues over the lungs of healthy mice.

“The dramatic increase indicated that these molecules were doing something to the cancer – but we didn’t know if it was harmful or good. We asked, are they trying to stop the cancer, or facilitating it? So we studied the individual properties and saw that they are working against the cancer in several ways.”

Das said.

Slowed Tumor Growth

The researchers found that in higher concentrations, EDP-EAs did kill cancer cells, but not as effectively as other chemotherapeutic drugs on the market.

However, the compounds also combated the osteosarcoma in other ways: They slowed tumor growth by inhibiting new blood vessels from forming to supply the tumor with nutrients, they prevented interactions between the cells, and most significantly, they appeared to stop cancerous cells from migrating.

“The major cause of death from cancer is driven by the spread of tumor cells, which requires migration of cells. As such, therapies that have the potential to impede cell migration also could be useful for slowing down or inhibiting metastases,”

said study coauthor Timothy Fan, a professor of veterinary clinical medicine and veterinary oncology.

Comparative biosciences professor Aditi Das and veterinary clinical medicine professor Timothy Fan

Comparative biosciences professor Aditi Das and veterinary clinical medicine professor Timothy Fan
Credit: L. Brian Stauffer

The researchers isolated the most potent of the molecules and are working to develop derivatives that bind better to the cannabinoid receptor, which is plentiful on the surface on cancer cells.

Omega-3 Fatty Acids

“Dietary consumption of omega-3 fatty acids can lead to the formation of these substances in the body and may have some beneficial effects. However, if you have cancer, you want something concentrated and fast acting. That’s where the endocannabinoid epoxide derivatives come into play – you could make a concentrated dose of the exact compound that’s most effective against the cancer. You could also mix this with other drugs such as chemotherapies,”

Das said.

Next, the researchers plan to perform preclinical studies in dogs, since dogs develop osteosarcoma spontaneously, similarly to humans. They also plan to study the effects of EDP-EAs derived from omega-3 fatty acids in other cancer types.

“Particular cancers that might be most interesting to study would be solid tumors or carcinomas, which tend to spread and cause pain within the skeleton. Some of the most common tumors that behave this way are breast, prostate, and lung carcinomas, and we can certainly explore these tumors in the future,”

said Fan, who is also a member of the Carle Illinois College of Medicine, the Cancer Center at Illinois and the Carl R. Woese Institute for Genomic Biology.

Jahnabi Roy, Josephine E. Watson, In Sup Hong, Timothy M. Fan, and Aditi Das
Antitumorigenic Properties of Omega-3 Endocannabinoid Epoxides
Journal of Medicinal Chemistry 2018 61 (13), 5569-5579 DOI: 10.1021/acs.jmedchem.8b00243

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