Methylene Blue Could Slow Skin Aging Symptoms

An antioxidant called methylene blue could slow or reverse several well-known signs of aging of human skin, new work from the University of Maryland suggests.

The researchers tested methylene blue, a common, inexpensive and safe chemical, for four weeks in skin cells from healthy middle-aged donors, as well as those diagnosed with progeria — a rare genetic disease that mimics the normal aging process at an accelerated rate. In addition to methylene blue, the researchers also tested three other known antioxidants: N-Acetyl-L-Cysteine (NAC), MitoQ and MitoTEMPO (mTEM).

“Our work suggests that methylene blue could be a powerful antioxidant for use in skin care products. The effects we are seeing are not temporary. Methylene blue appears to make fundamental, long-term changes to skin cells,”

said Kan Cao, senior author on the study and an associate professor of cell biology and molecular genetics at UMD.

Range Of Improvements

In their experiments, methylene blue outperformed the other three antioxidants, improving several age-related symptoms in cells from both healthy donors and progeria patients. The skin cells (fibroblasts, the cells that produce the structural protein collagen) experienced a decrease in damaging molecules known as reactive oxygen species, a reduced rate of cell death and an increase in the rate of cell division throughout the four-week treatment.

Next, Cao and her colleagues tested methylene blue in fibroblasts from older donors (>80 years old) again for a period of four weeks. At the end of the treatment, the cells from older donors had experienced a range of improvements, including decreased expression of two genes commonly used as indicators of cellular aging: senescence-associated beta-galactosidase and p16.

“I was encouraged and excited to see skin fibroblasts, derived from individuals more than 80 years old, grow much better in methylene blue-containing medium with reduced cellular senescence markers. Methylene blue demonstrates a great potential to delay skin aging for all ages,”

said Zheng-Mei Xiong, lead author of the study and an assistant research professor of cell biology and molecular genetics at UMD.

Methylene Blue On Simulated Skin

The researchers then used simulated human skin (a system developed by Cao and Xiong) to perform several more experiments. This simulated skin — a three-dimensional model made of living skin cells — includes all the major layers and structures of skin tissue, with the exception of hair follicles and sweat glands.

The model skin could also be used in skin irritation tests required by the Food and Drug Administration for the approval of new cosmetic products, Cao said.

“This system allowed us to test a range of aging symptoms that we can’t replicate in cultured cells alone,” Cao said. “Most surprisingly, we saw that model skin treated with methylene blue retained more water and increased in thickness—both of which are features typical of younger skin.”

The researchers also used the model skin to test the safety of cosmetic creams with methylene blue added. The results suggest that it causes little to no irritation, even at high concentrations. Encouraged by these results, Cao, Xiong and their colleagues hope to develop safe and effective ways for consumers to benefit from the properties of the antioxidant.

Zheng-Mei Xiong, Mike O’Donovan, Linlin Sun, Ji Young Choi, Margaret Ren & Kan Cao
Anti-Aging Potentials of Methylene Blue for Human Skin Longevity
Scientific Reports 7, Article number: 2475 (2017) doi:10.1038/s41598-017-02419-3