Lateral Amygdala Fear Learning Controlled By Central Amygdala

The central amygdala, rather than the lateral amygdala, is where aversive learning is regulated, new research from Cold Spring Harbor Laboratory suggests.

The finding, if confirmed, could change approaches to modify memory to treat disorders such as anxiety and post-traumatic stress disorder (PTSD).

Textbooks indicate that the lateral portion of the amygdala is the place where associations are forged between information provided by the senses and the perception of threat. That linkage is a crucial example of learning – what scientists call aversive learning.

Lateral Amygdala

What happens in the brain when we learn that fire is very hot and can hurt us? It’s the kind of learning that results from the association of a sensory stimulus and the perception of threat.

A neuroscience team at Cold Spring Harbor Laboratory (CSHL) led by Professor Bo Li studied the relationship between two parts of the brain’s amygdala – an almond-shaped structure that comes in pairs, one situated on each side of the mouse and other vertebrate brains. The amygdalae are centrally involved in learning and memory.

One series of experiments involved giving a mild foot shock to mice and then imaging the central and lateral parts of the amygdala. A fluorescent protein that registers when cells are active showed that the response to an uncomfortable feeling begins in the central amygdala, then registers in the lateral amygdala.

In other experiments, the team blocked the activity of a particular set of neurons in the central amygdala – named for a protein they express called PKC-delta (protein kinase C-delta). When mice with this impairment were given a foot shock, there was greatly attenuated activity in the lateral amygdala.

“If the conventional view was correct, you should see normal levels of activity in the lateral amygdala – where the activity supposedly begins. But this wasn’t the case,”

says Li.

PKC-delta Neurons

A final set of experiments had the intriguing aim of giving mice artificial memories – memories of discomfort like that of getting a mild shock. This is done using optogenetics, a technology in which pulses of colored laser light are used to activate specific neurons, in this case the PKC-delta neurons of the central amygdala.

“This causes no pain to the mice, but they don’t like it – it makes them feel uncomfortable, just as getting a mild shock makes them uncomfortable. It’s the kind of stimulation that leads to an aversive memory, the kind you learn from,”

explains Li. In effect, the scientists create a memory of a discomfort never actually experienced.

The experiment shows that activation of the PKC-delta cells in the central amygdala is indeed at the heart of the aversive learning process.

This was concluded after placing the mice given the optogenetic stimulus into a cage with two chambers. Mice that received the stimulation “remembered” the discomfort and associated it with the place where they experienced it – one of the two chambers.

Later, given the freedom to move about in this space, none of the “treated” mice would venture back to the chamber where the stimulation was given.

“It appears to be a stable memory,” says Li. “After several days, we saw them continue to avoid the place associated with the discomfort.”

Among the take-away lessons of these experiments is that PKC-delta neurons play an important role in conveying information about unexpected discomfort from the central amygdala to the lateral amygdala. The research suggests that this subset of central amygdala neurons are “fear-on” cells.

Li and his team hope that this information will inform ongoing attempts to find new ways to alter fear memories that are based on spurious associations – for example, a combat veteran freezing in fear or experiencing panic at the sound of a backfiring car.

The research was supported by grants from the National Institutes of Health, Human Frontier Science Program, NARSAD, National Natural Science Foundation of China, China Postdoctoral Science Foundation, Louis Feil Trust, the Stanley Family Foundation, Simons Foundation, and Wodecroft Foundation.

Kai Yu, Sandra Ahrens, Xian Zhang, Hillary Schiff, Charu Ramakrishnan, Lief Fenno, Karl Deisseroth, Fei Zhao, Min-Hua Luo, Ling Gong, Miao He, Pengcheng Zhou, Liam Paninski & Bo Li
The central amygdala controls learning in the lateral amygdala
Nature Neuroscience (2017) doi:10.1038/s41593-017-0009-9

Image: PKC delta-expressing cells, located in the brain’s central amygdala. Credit: Li Lab CSHL