A Bidirectional Model Of Cerebellar Learning

A new model of the molecular basis of learning in the cerebellum, a part of the brain that receives sensory input and coordinates voluntary movements, has been developed by researchers at the Okinawa Institute of Science and Technology Graduate University (OIST).

The process we call learning is in fact a well-orchestrated symphony of thousands of molecular reactions, but the exact interplay between these reactions remains largely unknown.

“As far as we know, this is the most complex model of such a system that exists,”

said senior author Erik De Schutter, head of OIST’s Computational Neuroscience Unit. Previous models focused on the signals that arrive at the receiving end of a neuron, but, he said, now scientists are looking at the ongoing communication between the two ends.

LTP And LTD

Learning is thought to be a balance between two processes that act as a kind of molecular dial: long-term potentiation (LTP), in which the connection between two neurons is strengthened, and long-term depression (LTD), in which the connection between two neurons is weakened. Both these processes take place at the synapse — the junction between two neurons.

Andrew Gallimore, first author on the paper and a postdoctoral researcher at OIST, modeled how they work in two types of cells: parallel fibers and Purkinje cells, which play a key role in motor learning.

Using a computer program to create a model of this complex system, Gallimore combined several hundred equations taken from experiments in which such neurons were activated.

Unified bidirectional model of cerebellar parallel fiber-Purkinje cell LTD and LTP

Credit: OIST Computational Neuroscience Unit

The model was put to the test when colleagues in Korea took recordings from neurons in the cerebellum of mice. The OIST researchers then incorporated these recordings into the model.

Their findings show that the molecular networks on both sides of a synapse are important for controlling learning: communication must occur in both directions across the synapse to control whether LTD or LTP is generated during neural activity.

Molecular Balancing Act

The model also showed that the molecular dial balancing LTP and LTD has an automatic off-switch that, when triggered, allows the system to return to its resting state. Although previous research hinted at the presence of this off-switch, this is the first time that the mechanism behind it — a complex network of proteins and receptors — has been demonstrated.

Such a large, comprehensive model allows scientists to examine how complex signaling systems work together, something that is often absent in experimental literature, De Schutter said.

The researchers’ work allows scientists to more accurately predict the behavior of the chaotic, complex system of molecules that controls learning. It also hints at what might be happening at the molecular level when these switches break — which might occur when the brain is injured or during neurodegenerative diseases that affect learning.

“The whole function of a brain is based on the strengths of these synaptic connections. The better we understand these processes, the greater potential there is to intervene to mitigate severe problems,”

said Gallimore.

Gallimore, Andrew R. et al.
Switching On Depression and Potentiation in the Cerebellum
Cell Reports, Volume 22, Issue 3, 722 – 733

Top Image: parallel fiber-Purkinje cell. The presynaptic cell, a parallel fiber, is colored red while the postsynaptic cell, a Purkinje cell, is colored green. Credit: OIST Computational Neuroscience Unit