Suppression Of Incongruous Directional Signals Prevents Axon Misguidance

Motor neurons receive a multitude of cues to successfully navigate to their destinations in the body, report Salk Institute scientists.

“There are 100 trillion connections in the nervous system governed by 20,000 genes, of which roughly 10 gene families are known to be involved in controlling axon guidance. We wanted to understand the clever genetic systems nature has employed to wire the most complicated biological machine in the universe. Thus, we set out to examine how motor neurons find their connections with muscles in the body, which is critical for our brain to relay information to our muscles to allow for movement,”

says Salk Professor Samuel Pfaff, senior author and a Howard Hughes Medical Institute investigator.

Axon Guidance Signals

The brain controls hundreds of different muscles to allow for precise movement. During development, motor neurons in the spinal cord extend their axons outside of the central nervous system to connect with muscle cells in the body.

Every motor neuron relies on a set of genes to ensure the axon grows correctly to the muscle.

“In the broadest sense, we hoped that by identifying genes involved in abnormal motor neuron development we could better understand the intricacies of cell signaling in other contexts such as cancer. This work not only shows us how the nervous system develops, but also how cells, more generally, communicate, move and create structures in the body,”

says first author Dario Bonanomi, a former postdoctoral fellow in the Pfaff lab, now a group leader at the San Raffaele Scientific Institute.

Wrong Detour

To find genes key to motor-neuron axon guidance, the team cconducted a genetic screen and observed where the motor neurons grew their axons using green fluorescent proteins in a mouse model they engineered. The team then traced the axons to see when correct and incorrect growth decisions were made.

Through this axon tracing, the scientists identified a gene mutation that caused motor axon misrouting. In this case, the motor neuron axons took detours and never connected properly to the muscles.

Upon closer investigation, the scientists found that these motor neurons climbed up the edge of the spinal cord rather than properly exiting to meet their muscle targets. The team pinpointed the gene causing this detrimental mutation as p190, which was previously known to play a role in cancer suppression, but had not been implicated in establishing neuronal connections during development.

Context-dependent Gate

The researchers set up a series of experiments to examine how p190 affects axons leaving the spinal cord. They found that, although axons are normally attracted to a protein in the spinal cord called netrin, during a short time window p190 acts as a blinder, so the axons disregard netrin and are led outside of the spinal cord.

After the axons safely leave the central nervous system, this blinder is removed. Without p190, the axons are attracted to netrin and do not properly leave the spinal cord, so never connect with muscles.

Pfaff, holder of the Benjamin H. Lewis Chair, adds,

“These results provide mechanistic insight into the unimagined complexity that cells use to communicate with one another.”

The next step is to examine the control mechanism of p190, and what factors impact the timing of its activity, say the researchers. The research was supported by the Howard Hughes Medical Institute, the Sol Goldman Trust, the European Research Council, and a Career Development Award from the Giovanni Armenise-Harvard Foundation.

Dario Bonanomi, Fabiola Valenza, Onanong Chivatakarn, Matthew J. Sternfeld, Shawn P. Driscoll, Aaron Aslanian, Karen Lettieri, Miriam Gullo, Aurora Badaloni, Joseph W. Lewcock
p190RhoGAP Filters Competing Signals to Resolve Axon Guidance Conflicts
Neuron, March 19, 2019, DOI:https://doi.org/10.1016/j.neuron.2019.02.034

Image:  Salk Institute. Left: Motor neurons (green) exit the spinal cord (red) and enter the periphery of the body to connect with muscles. Right: Motor neurons (white arrow) without the guidance of p190 are trapped within the spinal cord.