Fasting Activates Genetic Switch That Helps Stop Inflammation
A new study suggests that a molecular pathway activated in the brain during fasting helps halt the spread of intestinal bacteria into the bloodstream.
The study, by a team of researchers at the Salk Institute, demonstrates a molecular pathway by which the brain communicates with the gastrointestinal (GI) tract to prevent unnecessary activation of the immune system during fasting by strengthening the barrier against gut microbes. The finding of this brain-gut signal in fruit flies, which have many similarities to humans, could impact the treatment of inflammatory bowel diseases in people.
The GI tract, in addition to its role in promoting the absorption of nutrients from food, is host to a range of bacteria. These microbes help in the digestive process by producing chemicals that break down complex fats and carbohydrates.
Lead investigator Marc Montminy, professor in the Clayton Foundation Laboratories for Peptide Biology, said:
“Fasting has a positive value that spills over not just into the metabolic system, but also inflammation and brain function. Understanding how the gut maintains this barrier, and creating drugs to enhance that barrier, may have important benefits for people with inflammatory bowel disease.”
Crtc and CREB
The new study is part of an ongoing collaborative effort by the Montminy lab and the lab of Salk Professor John Thomas to pin down the mechanisms that a genetic switch in the brain called Crtc uses to control energy balance.
A constant network of communication between our brains and the GI tract, as well as other tissues, helps our bodies keep tabs on our energy expenditure and stores. Crtc interacts with another molecule called CREB, and fasting activates both proteins and boosts formation of long-term memories.
The Montminy and Thomas teams used fruit flies to study the Crtc switch, in part because flies express many of the same metabolism-related genes as humans do.
Previous experiments by the two labs have shown that flies whose Crtc gene is deleted become sensitized to fasting; they only survive about half as long without food compared to flies with the Crtc gene. The researchers wanted to understand why the deletion of Crtc caused flies to die sooner and had hypothesized it was because these mutant flies have fewer fat and sugar stores.
What they found in the study, however, was surprising and more complicated.
The guts of the flies without Crtc expressed several molecules indicating that their immune system was keyed up. When postdoctoral researcher Run Shen entered Montminy’s lab with the evidence—pictures taken from the microscope of fluorescently stained cells lining the flies’ guts—”it was totally unexpected,” he says.
What does this mean?
The new results suggest that the flies are more sensitive to starvation because the immune system is activated, which is energetically taxing. This amped-up immune response suggests that without Crtc, bacteria leak from the gut into the fly’s circulation.
The researchers found that the normal role of Crtc is to fortify the barriers of the gut to prevent bacteria from entering the bloodstream and awakening the immune system. Without Crtc, the connections between cells that line the gut tube become disrupted, causing bacteria to leak out, activating the immune response and depleting energy reserves.
Short Neuropeptide F
While looking for molecular partners of Crtc, the researchers uncovered a protein called short neuropeptide F (sNPF), which is also found in the brain and has an equivalent in humans called neuropeptide Y.
This compound is known to cause flies and mammals to search for food in response to hunger signals. Without sNPF in the brain, the flies showed signs of gut inflammation similar to those flies missing Crtc.
What’s more, the normally tight seals along the gastrointestinal tract were broken down in the sNPF-lacking flies, letting bacteria out.
Conversely, flies expressing more than the normal amounts of Crtc or sNPF in their neurons were able to survive longer without food and showed less disruption to the tight junctions that maintain their gastrointestinal barriers.
The researchers are conducting more experiments to understand how the neuropeptides activate the gut receptors that help protect it from bacterial invasion.
Neuronal energy-sensing pathway promotes energy balance by modulating disease tolerance PNAS, www.pnas.org/cgi/doi/10.1073/pnas.1606106113
Image: The gastrointestinal tract of flies lacking Crtc, which is turned on during fasting (right panel), expresses more immune-related molecules compared with those of normal flies (left panel). These signs of inflammation mean that the gut barrier is compromised and more permeable to bacteria. Credit: Salk Institute