Disrupted Dopamine Signaling Holds Clue To Autism

Recently discovered genetic variations linked to autism spectrum disorder alter the function of the dopamine transporter, according to a Vanderbilt University led research team. The findings suggest that altered dopamine signaling contributes to this common developmental condition.

Two distict inherited genetic variations in two different families of children with autism spectrum disorder (ASD) “converged” to produce the same changes in nerve function and behavior, the researchers report.

This is the first report of two different ASD-associated variations producing the same neural and behavioral changes in a Drosophila (fruit fly) model, said Aurelio Galli, Ph.D., professor of Molecular Physiology and Biophysics and of Psychiatry, and corresponding author with Kevin Erreger, Ph.D., and Heinrich J.G. Matthies, Ph.D.

Disrupted Dopamine Neurotransmission

The researchers are now developing a mouse model to confirm these findings, and they are working with biopharmaceutical firms to develop more effective treatments for ASD that specifically target the dopamine transporter, he said.

“We’re looking at a network of molecules that disrupt dopamine neurotransmission in different ways, but they converge sometimes with the same mechanism,” Galli said. “We’ve never seen anything like that before.”

Dopamine is a neurotransmitter involved in reward behaviors, including appetite, addiction and social interaction.

Normally the dopamine transporter “sweeps up” excess dopamine from the synapse or gap between nerve cells. Amphetamine paradoxically reduces hyperactive behaviors in children with ASD by causing the transporter to work in “reverse,” and thus increase the supply of dopamine in the synapse.

Syntaxin 1 Gene

Working with James Sutcliffe, Ph.D., associate professor of Molecular Physiology and Biophysics and of Psychiatry, the researchers identified inherited variations in the dopamine transporter gene and in the gene for syntaxin 1 (STX1), a protein that regulates the transporter, in two families of children with ASD.

Mimicking the molecular properties of either of the two mutations in the Drosophila fly model disrupted amphetamine-induced locomotion, demonstrating a reduced behavioral response to the drug amphetamine, said Galli, whose team in 2013 reported the first model of a spontaneously arising mutation in the dopamine transporter associated with neuropsychiatric disorders.

Locomotion is a good model for reward behaviors, he said, because you have to move to get food; you have to move to do anything you like.

“We are starting to look at the function of the dopamine transporter in a completely different way,” Galli said. “We understand molecularly what is happening to the dopaminergic system and how now to target it.”

Etienne Cartier, Peter J. Hamilton, Andrea N. Belovich, Aparna Shekar, Nicholas G. Campbell, Christine Saunders, Thorvald F. Andreassen, Ulrik Gether, Jeremy Veenstra-Vanderweele, James S. Sutcliffe, Paula G. Ulery-Reynolds, Kevin Erreger, Heinrich J.G. Matthies, Aurelio Galli.
Rare Autism-Associated Variants Implicate Syntaxin 1 (STX1 R26Q) Phosphorylation and the Dopamine Transporter (hDAT R51W) in Dopamine Neurotransmission and Behaviors.
EBioMedicine, 2015; DOI: 10.1016/j.ebiom.2015.01.007

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