Abraxane Drives Macrophage Activation In Pancreatic Cancer

Abraxane (nab-paclitaxel), a drug created to prevent cancer cells from multiplying has a secondary effect, researchers from NYU Langone Medical Center have discovered. It also switches immune cells that turn down the body’s attack on tumors back into the kind that amplify it.

Macrophages – immune cells that home in on tumors – take in the drug Abraxane, say the study authors. Once inside these cells, the drug changes them so that they signal for an aggressive anti-tumor immune response, according to experiments in mice.

Corresponding author Dafna Bar-Sagi, PhD, Vice Dean for Science and Chief Scientific Officer at NYU Langone, says:

“Our study reveals a previously unappreciated role for Abraxane in tumor immunology. In doing so, it suggests ways to improve the drug and argues for its inclusion in new kinds of combination treatments.”

Abraxane, Paclitaxel And Albumin

Abraxane is comprised of the decades-old cancer drug, paclitaxel, combined with nanoparticles of the protein albumin (nab). Paclitaxel alone is not effective against pancreatic cancer, but Abraxane (nab-paclitaxel) is part of a leading treatment for the disease.

Why the albumin-bound form works better has been a major question in the field.

Paclitaxel prevents structures called microtubules inside cancer cells from breaking up, a required step if they are to multiply as part of abnormal growth. Many in the field assume that nab-paclitaxel too primarily targets microtubules in cancer cells, with albumin perhaps helping the drug to get inside cells, and with fewer toxic side-effects.

The new findings suggest that, on top of any effect on cancer cells, Abraxane’s effectiveness may proceed from its impact on macrophages, which roam the bloodstream and build up in many tumors.

The study results revolve around the immune system, in which cells like macrophages trigger a massive attack on bacteria or other invading microbes. This system can also recognize and attack cancer cells.

Factors secreted by tumor cells, however, dampen the immune response in part by switching macrophages from their immune-stimulating stance, termed M1, into an M2 mode that suppresses their immune function.

Macropinocytosis

In experiments in macrophage cell lines, the study authors found that nab-paclitaxel is more effective than paclitaxel partly because albumin enables macrophages to take up the drug through a natural process called macropinocytosis.

Lead author Jane Cullis, PhD, a postdoctoral fellow in Bar-Sagi’s lab, says:

“Our findings argue that it may be possible to develop more treatments that selectively target macrophages by coupling albumin to immune-activating agents. We may also be able to adjust albumin’s structure such that drugs attached to it stay in macrophages longer, or combine Abraxane with T-cell treatments for greater therapeutic effect. In principle, such treatments should be useful against the many tumor types infiltrated by macrophages.”

Once inside macrophages, according to experiments in mice with pancreatic tumors, nab-paclitaxel causes the macrophages to switch from immune-suppressing M2 cells back into M1 cells that amplify the body’s effort to kill cancer cells.

Past studies had found that paclitaxel has a similar structure to substances given off by bacteria that trigger macrophage activation. The study authors show that the same pathway is evoked by nab-paclitaxel in pancreatic tumor-associated macrophages.

Jane Cullis et al
Macropinocytosis of Nab-paclitaxel Drives Macrophage Activation in Pancreatic Cancer
Cancer Immunology Research (2017). DOI: 10.1158/2326-6066.CIR-16-0125

Image: Michael Bright, Wellcome Images. Scanning electron micrograph of a macrophage phagocytosing an IgG-coated erythrocyte.

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